Many undergraduate and graduate students also use this statistical program. Meanwhile Graphpad Prism is used throughout the life sciences sector. GraphPad Prism was originally developed for experimental biologists, medicine scientists and pharmacologists. Even without previous knowledge, statistical tests can simply be executed and their results can easily be interpreted.
Because most of the data was distributed not normally, results are expressed as median and interquartile range (IQR) as dispersion characteristic, and p value less than 0.05 ( p < 0.05) was considered as statistically significant.Your scientific data from experiments and observations are easy to organize, analyze and visualized with the help of the software GraphPad Prism. If data were not normally distributed, the comparison of medians between different groups was switched to non-parametric one-way ANOVA on ranks or Kruskal-Wallis test followed by two-stage step-up method of Benjamini, Krieger and Yekutieli as post-hoc test. Homogeneity of variances was tested using Brown-Forsythe and Bartlett’s tests, and in a case of unequal SDs Brown-Forsythe and Welch ANOVA test was applied. The comparison of means between different groups of numerical variables was performed using one-way ANOVA. Z-value equal or higher than 2, also equal or lower than −2 indicates that variable significantly differs from the control group mean. Z-transformation of the QST data was performed using the following expression z = ( x − μ)/ σ, where x corresponds to the individual variable from the datasets that must be normalized with z-transformation, while μ and σ are the control group mean and standard deviation (SD), respectively. To test whether the collected numerical data are normally distributed, the D’Agostino and Pearson, Anderson-Darling and Shapiro-Wilk normality tests were applied.
All the graphs, calculations, and statistical analyses were performed using GraphPad Prism software version 8.0 for Mac (GraphPad Software, San Diego, CA, USA).
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